Measurement
14QE
Part:BBa_I744102:Design
Designed by: Thomas Segall-Shapiro Group: iGEM07_Rice (2007-07-18)
Tetracycline sensor with medium-strength promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The promoter driving tetR production is crucial to the sensor's sensitivity and range of detection. In ths case, the promoter is weakly constitutive, but stronger than the promoter in 'BBa_I744101'. Hence, the sensor should be slightly less sensitive but still dependent on copy number.
Source
Composite - all parts are taken from the registry