Measurement
14QE

Part:BBa_I744102:Design

Designed by: Thomas Segall-Shapiro   Group: iGEM07_Rice   (2007-07-18)


Tetracycline sensor with medium-strength promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter driving tetR production is crucial to the sensor's sensitivity and range of detection. In ths case, the promoter is weakly constitutive, but stronger than the promoter in 'BBa_I744101'. Hence, the sensor should be slightly less sensitive but still dependent on copy number.

Source

Composite - all parts are taken from the registry

References